Journal: iScience

Article Title: C5aR1-positive adipocytes mediate non-shivering thermogenesis in neonatal mice

doi: 10.1016/j.isci.2024.111261

Figure Lengend Snippet:

Article Snippet: PVDF membrane blots were blocked in 5% skimmed milk for 1h at room temperature, washed in Tris-buffered saline with Tween 20 (TBST) and incubated overnight at 4°C with rabbit anti-UCP1 (1:1000, Sigma, U6382), anti-C5aR1 (1:500, Abcam, ab59390), anti-PPARγ (1:1000, CST, 2435), anti-β-ACTIN (1:5000, ABclonal, AC038), anti-HSP90 (1:1000, ABclonal, A5027), Mouse anti-α-TUBLIN (1:200000, Proteintech, 66031-1-Ig).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, SYBR Green Assay, Sequencing, Software

Journal: Acta Pharmaceutica Sinica. B

Article Title: (+)/(−)-Gerbeloid A, a pair of unprecedented coumarin-based polycyclic meroterpenoid enantiomers from Gerbera piloselloides : Structural elucidation, semi-synthesis, and lipid-lowering activity

doi: 10.1016/j.apsb.2024.03.035

Figure Lengend Snippet: (+)- 1a and (−)- 1b regulated the expression of adipogenic and lipogenic proteins in 3T3-L1 adipocytes. The 3T3-L1 adipocytes were incubated with a control vehicle or (+)- 1a and (−)- 1b (12.5, 25, and 50 μmol/L) for 6 days. (A) Expression of proteins involved in adipogenic differentiation and lipid accumulation (PPAR γ and C/EBP α ) was determined by Western blot, vinculin was used as a loading control. (B) Expression of proteins involved in adipocyte lipolysis (p-PKA, HSL, p-HSL 563 , p-HSL 660 , and Perilipin) was detected by Western blot, vinculin was used as a loading control. The data are expressed as mean ± SD ( n = 3). ### P < 0.001 vs vehicle, ns P > 0.05, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs IDIR.

Article Snippet: Fetal bovine serum (10099-141), Dulbecco′s modified Eagle medium (8122175), Pen-streptomycin (15140-122), Typsin-EDTA (25200072) were purchased from Gibco (Cambridge, USA); Anti-PKA (ab75991) was purchased from Abcam (Cambridge, UK); PPAR γ (2435T), C/EBP α (8178T), Vinculin (13901S) and Lipolysis activation antibody sampler kit (8334T) were purchased from Cell signaling technology (Danvers, USA).

Techniques: Expressing, Incubation, Control, Western Blot

Journal: Microbial Genomics

Article Title: Insights into microbial dysbiosis and Cutibacterium acnes CAMP factor interactions in acne vulgaris

doi: 10.1099/mgen.0.001449

Figure Lengend Snippet: CAMP factors trigger acne-like lesions in mice. (a, b) Representative overviews (scale bar: 0.2 cm) and HE staining (scale bar: 100 µm) of dorsal skin treated with CAMP proteins or PBS ( n =4). (c) RT-qPCR analysis of mRNA levels of acne-characteristic factors. Statistical significance was calculated using one-way ANOVA ( n =3, **** P <0.0001, *** P <0.001, ** P <0.01; ns, not significant). (d) Immunofluorescence staining and quantification of PPAR γ and K10 expression in mouse dorsal skin (scale bar: 100 µm; n =3; **** P <0.0001, *** P <0.001, ** P <0.01).

Article Snippet: Immunofluorescence staining was performed using PPAR γ (Cell Signaling, 2435T) and K10 (Abcam, ab76318) antibodies, as described previously [ ].

Techniques: Staining, Quantitative RT-PCR, Immunofluorescence, Expressing